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Updated: Jun 4, 2026
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High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
Published on: October 19, 2013
Whole mount RNA in situ hybridization on zebrafish embryos: mounting
1Division of Basic Science, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
CSH Protocols
|March 2, 2011
Abstract:
INTRODUCTIONThis protocol describes the mounting of zebrafish embryos for observation after whole-mount in situ hybridization. Embryos can be mounted in glycerol or dehydrated and mounted in Permount, with or without the yolk. Glycerol causes less shrinkage, but is not as effective a clearing agent. Embryos mounted on the yolk should be stored in the dark and photographed immediately, because the yolks darken rapidly.
