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Updated: Jun 4, 2026
Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling
Published on: May 31, 2017
Abstract:
INTRODUCTIONPeptide mapping by MALDI-TOF-MS has emerged as a powerful proteomics tool for identifying and characterizing proteins. One of the key steps in this method involves the proteolytic cleavage of proteins, followed by MS analysis of the generated peptides. A limitation of the method, especially for high-throughput proteomics, is the speed and efficiency of proteolytic cleavage. In this protocol, a method for rapid tryptic digestion using immobilized enzyme microcolumns is described.
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