Maintaining a population of Drosophila

INTRODUCTIONThe Drosophila embryo is a superb source of proteins for studies aimed at characterizing a variety of cellular functions, including replication, transcription, translation, signal transduction, and the functions of the extracellular matrix and the cytoskeleton. The success of these studies requires the efficient production of gram quantities of embryos by large populations of adult flies. This protocol outlines a simple and efficient method for maintaining a population of Drosophila.