Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 4, 2026

Micromanipulation of Chromosomes in Insect Spermatocytes
05:45

Micromanipulation of Chromosomes in Insect Spermatocytes

Published on: October 22, 2018

Embryo dissection and micromanipulation tools.

Hazel L Sive, Robert M Grainger, Richard M Harland

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sleeping upside-down: Knockdown of a sleep-associated gene induces daytime sleep in the jellyfish <i>Cassiopea</i>.

    Proceedings of the National Academy of Sciences of the United States of America·2025
    Same author

    Modelling human genetic disorders in Xenopus tropicalis.

    Disease models & mechanisms·2024
    Same author

    A time-resolved single-cell roadmap of the logic driving anterior neural crest diversification from neural border to migration stages.

    Proceedings of the National Academy of Sciences of the United States of America·2024
    Same author

    "And it was the worst possible result, because it actually worked:" An interview with Richard Harland.

    Cells & development·2024
    Same author

    Conserved chromatin and repetitive patterns reveal slow genome evolution in frogs.

    Nature communications·2024
    Same author

    Erratum: Best Practices for <i>Xenopus tropicalis</i> Husbandry.

    Cold Spring Harbor protocols·2023
    Same journal

    Identification of positive GATEWAY expression clones when both the pENTRY and pDEST vectors contain the same marker for bacterial selection.

    CSH protocols·2012
    Same journal

    Imaging protein interactions by FRET microscopy: cell preparation for FRET analysis.

    CSH protocols·2012
    Same journal

    Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes.

    CSH protocols·2012
    Same journal

    Bradford assay.

    CSH protocols·2012
    Same journal

    Detection of ubiquitylated proteins in mammalian cells.

    CSH protocols·2012
    Same journal

    Imaging of organelle membrane systems and membrane traffic in living cells.

    CSH protocols·2012
    See all related articles

    This article details the creation and upkeep of specialized tools for embryo dissection and micromanipulation. Maintaining sterile conditions with 70% ethanol is crucial for tool longevity and experimental integrity.

    Area of Science:

    • Developmental Biology
    • Laboratory Techniques

    Background:

    • Micromanipulation and dissection of embryos are critical for developmental biology research.
    • Maintaining sterile laboratory tools is essential to prevent contamination and ensure accurate experimental outcomes.

    Purpose of the Study:

    • To describe the methods for creating and maintaining dissection and micromanipulation tools.
    • To emphasize the importance of sterile tool maintenance in embryo research.

    Main Methods:

    • Fabrication of specialized dissection and micromanipulation instruments.
    • Standardized cleaning and sterilization protocols using 70% ethanol.

    Main Results:

    • Successfully created functional and durable tools for delicate embryo procedures.

    More Related Videos

    Dissection of 6.5 dpc Mouse Embryos
    09:57

    Dissection of 6.5 dpc Mouse Embryos

    Published on: February 25, 2007

    Related Experiment Videos

    Last Updated: Jun 4, 2026

    Micromanipulation of Chromosomes in Insect Spermatocytes
    05:45

    Micromanipulation of Chromosomes in Insect Spermatocytes

    Published on: October 22, 2018

    Dissection of 6.5 dpc Mouse Embryos
    09:57

    Dissection of 6.5 dpc Mouse Embryos

    Published on: February 25, 2007

  • Established a reliable protocol for maintaining tool sterility.

    • Conclusions:

    • Proper tool creation and consistent sterile maintenance are vital for successful embryo micromanipulation and dissection.
    • The described methods ensure the longevity and efficacy of laboratory tools in sensitive biological research.