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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Updated: Jun 4, 2026

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

Published on: May 18, 2013

Preparing DNA from blood for genotyping.

Amrik Sahota¹, Andrew I Brooks, Jay A Tischfield

¹Rutgers University Cell and DNA Repository, Department of Genetics, Piscataway, NJ 08854-8082, USA.

|March 2, 2011

Summary

This summary is machine-generated.

This protocol details genomic DNA extraction from whole blood and buffy coats using various scales and kits. It also covers methods for compromised or clotted blood samples, ensuring DNA recovery.

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Last Updated: Jun 4, 2026

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Collection and Extraction of Saliva DNA for Next Generation Sequencing

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Area of Science:

Molecular Biology
Genetics

Background:

Genomic DNA extraction is fundamental for various molecular biology applications.
Standard protocols may not adequately address compromised or clotted blood samples.

Purpose of the Study:

To describe standardized protocols for genomic DNA extraction from whole blood and buffy coats.
To provide methods for handling compromised and clotted blood samples.
To outline scalable DNA extraction procedures for different sample volumes.

Main Methods:

Large-scale DNA extraction using in-house or commercial reagents (PUREGENE).
Mid-scale DNA extraction utilizing the QIAamp DNA Blood Midi Kit (0.5-mL samples).
Small-scale DNA extraction employing the QIAamp DNA Blood Mini Kits (200-μL samples).
Specific methods for compromised (e.g., transit, temperature-affected, improperly collected) and clotted blood samples.

Main Results:

The described methods are not high-throughput, with potential drawbacks in DNA recovery and data quality for high-throughput adaptations.
DNA yields are dependent on the number of nucleated cells and sample quality.
Successful DNA extraction is achievable from various blood sample conditions.

Conclusions:

This protocol offers versatile genomic DNA extraction methods suitable for diverse sample types and conditions.
The methods provide a reliable approach for obtaining genomic DNA from challenging blood samples.
Careful consideration of sample quality and protocol scale is essential for optimal DNA yield and quality.