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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: Jun 4, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

Genotyping by dideoxy resequencing.

¹Hubrecht Laboratorium, 3584 CT Utrecht, The Netherlands.

|March 2, 2011

Summary

This summary is machine-generated.

Dideoxy sequencing of PCR fragments offers a rapid genotyping method. This technique efficiently identifies heterozygous single-nucleotide polymorphisms (SNPs) in specific genomic regions.

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Last Updated: Jun 4, 2026

Pyrosequencing: A Simple Method for Accurate Genotyping

Pyrosequencing: A Simple Method for Accurate Genotyping

Published on: January 8, 2008

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

Molecular Biology
Genetics

Background:

Genotyping is crucial for genetic analysis.
Identifying single-nucleotide polymorphisms (SNPs) aids in understanding genetic variations.

Purpose of the Study:

To describe a protocol for dideoxy sequencing of PCR fragments.
To present a flexible genotyping technique for SNP identification.

Main Methods:

Utilizes dideoxy sequencing.
Applies Polymerase Chain Reaction (PCR) for fragment amplification.
Focuses on resequencing amplified DNA fragments.

Main Results:

Provides a quick method for genotyping.
Enables confirmation of heterozygous SNPs.
Applicable to specific genomic regions of interest.

Conclusions:

Dideoxy sequencing is an effective genotyping strategy.
This method is valuable for confirming the presence of heterozygous SNPs.