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Updated: Jun 4, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
Published on: April 28, 2022
Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination
Ming Lei1, Martin Winterhalder, Romedi Selm
1University of Konstanz, Department of Chemistry, D-78457 Konstanz, Germany.
Abstract:
A simple scheme for video-rate wide-field coherent anti-Stokes Raman scattering (CARS) microscopy is presented. The method is based on collinear nonphase-matching illumination. The mechanisms leading to CARS signal generation are investigated. We find that refraction-mediated phase-matching is the main effect. Video-rate wide-field CARS microscopy of polystyrene beads and CARS wide-field images of C. elegans embryos are shown, and the capabilities and the limitations of the scheme are discussed.
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