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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
Complementary DNA01:44

Complementary DNA

Overview

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Updated: Jun 4, 2026

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
06:59

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

Published on: June 26, 2019

Full-length transcriptome analysis using a bias-free cDNA library prepared with the vector-capping method.

Seishi Kato1, Mio Oshikawa, Kuniyo Ohtoko

  • 1Department of Rehabilitation Engineering, Research Institute, National Rehabilitation Center for Persons with Disabilities, Tokorozawa, Japan. kato-seishi@rehab.go.jp

Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2011
PubMed
Summary
This summary is machine-generated.

The vector-capping method efficiently prepares full-length complementary DNA (cDNA) libraries from minimal RNA. This technique ensures cDNA integrity and provides an unbiased representation of the cellular mRNA population for functional genomics research.

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Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
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Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

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Last Updated: Jun 4, 2026

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
06:59

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Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

Area of Science:

  • Molecular Biology
  • Functional Genomics
  • Biotechnology

Background:

  • Full-length complementary DNAs (cDNAs) are crucial for functional genomics studies.
  • Existing methods for cDNA library preparation can be inefficient or require substantial RNA input.
  • Ensuring the intactness and representative nature of cDNA libraries is vital for accurate downstream analysis.

Purpose of the Study:

  • To introduce a novel and efficient method for preparing full-length cDNA libraries.
  • To detail the protocol for the "vector-capping" method.
  • To highlight the advantages of the vector-capping method for cDNA integrity and representation.

Main Methods:

  • Development of the "vector-capping" method for cDNA library preparation.
  • Utilizing a small amount of total RNA as starting material.
  • Incorporating a quality control step to verify cDNA intactness via 5'-end sequence analysis.

Main Results:

  • The vector-capping method enables the preparation of full-length cDNA libraries with high efficiency.
  • The presence of dG at the 5' end of cDNA confirms its intactness.
  • The bias-free procedure ensures the cDNA library accurately reflects the cellular mRNA population.

Conclusions:

  • The vector-capping method is a simple, efficient, and reliable technique for generating high-quality full-length cDNA libraries.
  • This method is advantageous for functional genomics research, especially when RNA is limited.
  • The protocol facilitates accurate analysis of gene expression and function through representative cDNA libraries.