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Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications
Published on: April 21, 2016
Vapor-liquid-solid grown silica nanowire based electrochemical glucose biosensor
Eduardo Murphy-Pérez1, Sunil K Arya, Shekhar Bhansali
1Bio-MEMS and Microsystem Lab, Department of Electrical Engineering, University of South Florida, Tampa, FL 33620, USA.
The Analyst
|March 4, 2011
Summary
Silica nanowires enhance glucose oxidase immobilization on gold electrodes for biosensing applications. This method improves enzyme loading and detection sensitivity for glucose monitoring.
Area of Science:
- Nanomaterials Science
- Biosensors
- Electrochemistry
Background:
- Glucose oxidase (GOx) biosensors are crucial for glucose monitoring.
- Immobilization of enzymes on electrode surfaces is key to biosensor performance.
- Silica nanowires (SiO2NWs) offer a promising platform for enhanced enzyme immobilization.
Purpose of the Study:
- To develop a novel bioelectrode for glucose sensing using VLS-grown SiO2NWs.
- To investigate the covalent immobilization of GOx onto SiO2NWs.
- To characterize the electrochemical properties and performance of the fabricated bioelectrode.
Main Methods:
- Electrophoretic deposition of VLS-grown SiO2NWs on a gold electrode.
- Covalent immobilization of GOx using APTES and EDC/NHS chemistry.
- Characterization using SEM, TEM, and cyclic voltammetry.
- Electrochemical performance evaluation for glucose detection.
Main Results:
- SiO2NWs increased the effective electro-active surface area of the electrode.
- Higher loading of glucose oxidase was achieved via covalent immobilization.
- The fabricated bioelectrode exhibited a linear response in the range of 25-300 mg dL−1.
- A low detection limit of 11 mg dL−1 and high sensitivity of 0.463 µA (mg dL−1)−1 were achieved.
Conclusions:
- VLS-grown SiO2NWs provide an effective platform for GOx immobilization.
- The developed bioelectrode demonstrates excellent performance for glucose sensing.
- This approach holds potential for improved glucose monitoring devices.
