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Related Experiment Video

Updated: Jun 3, 2026

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Published on: May 22, 2020

HSV-Cellular Protein Interactions.

¹Department of Molecular Pathology, University College, London, UK.

Methods in Molecular Medicine

|March 5, 2011

Summary

Herpes simplex virus (HSV) gene expression relies on a specific sequence, involving viral proteins interacting with cellular transcription factors like Oct-1 and Spl for proper regulation during infection.

Area of Science:

Virology
Molecular Biology
Genetics

Background:

The herpes simplex virus (HSV) lytic cycle involves a strict temporal cascade of viral gene expression: immediate-early (IE), early, and late genes.
Viral regulatory proteins are crucial for this cascade, but they function by interacting with cellular transcription factors present in host cells.

Purpose of the Study:

To elucidate the intricate interplay between viral regulatory proteins and cellular transcription factors in controlling HSV gene expression during the lytic cycle.
To understand the role of specific cellular factors, such as Oct-1 and Spl, in the transactivation of viral IE genes.

Main Methods:

Analysis of viral gene promoter regions for binding sites of cellular transcription factors.
Investigating the interaction between virally encoded proteins (e.g., Vmw65) and cellular transcription factors (e.g., Oct-1, Spl).

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Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

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Last Updated: Jun 3, 2026

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Published on: May 22, 2020

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

Main Results:

The HSV virion protein Vmw65 requires complex formation with cellular Oct-1 and other factors to transactivate viral IE genes.
Cellular transcription factors like Spl bind to IE promoters, indicating their essential role in regulating viral gene expression.
Similar binding sites for cellular factors are found in promoters of other viral kinetic classes, including early genes like thymidine kinase.

Conclusions:

HSV gene expression regulation during the lytic cycle is a collaborative process heavily reliant on host cell transcription factors.
Understanding these viral-host interactions is key to deciphering the mechanisms of HSV infection and replication.