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Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
p53 inhibits mRNA 3' processing through its interaction with the CstF/BARD1 complex
F I Nazeer1, E Devany, S Mohammed
1Chemistry Department, Hunter College, City University of New York, New York, NY 10065, USA.
Oncogene
|March 9, 2011
Summary
The tumor suppressor p53 protein inhibits mRNA 3' cleavage during the DNA damage response. This novel function links RNA processing to the p53 network, impacting gene expression after UV exposure.
Area of Science:
- Molecular Biology
- Cancer Research
- Gene Regulation
Background:
- The precise mechanisms of p53-mediated gene expression control after DNA damage remain unclear.
- p53 is a critical tumor suppressor involved in cellular responses to DNA damage.
Purpose of the Study:
- To elucidate the role of p53 in regulating mRNA 3' cleavage during the DNA damage response.
- To investigate the interaction of p53 with factors involved in mRNA processing.
Main Methods:
- Co-immunoprecipitation assays to detect protein complexes.
- In vitro assays to assess p53's effect on mRNA 3' cleavage.
- Analysis of p53 mutations and their impact on protein interactions and RNA processing.
Main Results:
- p53 associates with BARD1 and CstF1, factors crucial for UV-induced inhibition of mRNA 3' cleavage.
- p53 inhibits mRNA 3' cleavage in vitro, with its expression inversely correlated to cleavage levels.
- A tumor-associated p53 mutation impairs interaction with BARD1/CstF and reduces UV-induced 3' processing inhibition.
Conclusions:
- p53 possesses a novel inhibitory function in 3' RNA processing, adding complexity to the DNA damage response.
- This function links RNA processing to the p53 network, influencing polyadenylation of specific genes.
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