Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

State-dependent energy conversion produces degenerate dissipation in active actomyosin networks.

bioRxiv : the preprint server for biology·2026
Same author

Mechanical movements generated by movable lipids break endosomal barriers for enhanced mRNA therapeutics.

Science advances·2026
Same author

A novel translation initiation codon single nucleotide variant of the SLC14A1 (c.2T>G) from a Chinese blood donor with Jk(a-b-) phenotype.

Transfusion·2026
Same author

A pico-calorimeter for cellular metabolism and antimicrobial susceptibility testing.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

High-fidelity compressed high-speed imaging for resolving rapid micro-dynamics.

Optics express·2026
Same author

Inverse-designed silicon nitride nanophotonics.

Nature communications·2026
Same journal

Multi-module collaborative optimization-driven fast speckle correlation imaging in variable environments.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Secrecy performance analysis of NOMA-UWOC systems over a vertically stratified WGG oceanic turbulence channel.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Backscattering of plane waves in a composite system containing a rough surface and anisotropic scatterers.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Aspherical surface construction methods based on extended Jacobi polynomials.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

OCT sidelobe suppression method based on dual-path phase sinusoidal modulation and minimum value fusion.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
Same journal

Optical design concepts using wavelength-selective diffractive optics to enable miniaturized multimodal endoscopic imaging across separated spectral ranges.

Journal of the Optical Society of America. A, Optics, image science, and vision·2026
See all related articles

Related Experiment Video

Updated: Jun 3, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Parallel two-step phase-shifting digital holograph microscopy based on a grating pair.

Peng Gao1, Baoli Yao, Irina Harder

  • 1State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.

Journal of the Optical Society of America. A, Optics, Image Science, and Vision
|March 9, 2011
PubMed
Summary
This summary is machine-generated.

A new optical setup enables parallel two-step phase-shifting digital holographic microscopy (DHM) for real-time phase imaging. This method uses a grating pair to create dual holograms, simplifying phase microscopy applications.

More Related Videos

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Related Experiment Videos

Last Updated: Jun 3, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Area of Science:

  • Optics
  • Microscopy
  • Holography

Background:

  • Phase-shifting digital holographic microscopy (DHM) is crucial for real-time phase imaging.
  • Existing DHM techniques can be complex and require specific optical setups.

Purpose of the Study:

  • To propose and implement a novel optical configuration for parallel two-step phase-shifting DHM.
  • To enable real-time phase microscopy with enhanced efficiency and reduced requirements.

Main Methods:

  • Utilizing a grating pair to diffract orthogonally circularly polarized object and reference waves twice.
  • Generating two parallel copies of each beam and combining them with polarization elements to obtain phase-shifting holograms.
  • Implementing two DHM schemes: slightly off-axis and on-axis.

Main Results:

  • The slightly off-axis DHM scheme effectively suppresses the dc term by hologram subtraction, reducing demands on the off-axis angle and CCD sampling power.
  • The on-axis DHM scheme minimizes requirements on CCD resolving power but necessitates pre-measurement of the reference wave intensity.
  • Both schemes demonstrate the viability of the proposed parallel two-step phase-shifting DHM configuration.

Conclusions:

  • The proposed grating-based optical configuration offers a versatile platform for parallel two-step phase-shifting DHM.
  • This approach facilitates real-time phase microscopy with adaptable schemes for different experimental constraints.
  • The developed methods provide practical solutions for advancing phase imaging capabilities in microscopy.