Related Experiment Video

Updated: Jun 3, 2026

Assessing Respiratory Immune Responses to Haemophilus Influenzae
06:32

Assessing Respiratory Immune Responses to Haemophilus Influenzae

Published on: June 29, 2021

[Immunobiologic characteristics of carbohydrate-containing preparations of Haemophilus influenzae]

O V Novikova, N G Kalina, N P Vaneeva

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |March 9, 2011
    PubMed

    Insights

    A novel Haemophilus influenzae type b (Hib) vaccine candidate, combining capsular polysaccharide (CPS Hib) with detoxified lipooligosaccharide (LOS NTHi), shows promise. This mixture demonstrates low toxicity and potent protective activity against Hib infections.

    Area of Science:

    • Immunology
    • Microbiology
    • Vaccine Development

    Background:

    • Haemophilus influenzae type b (Hib) is a significant pathogen.
    • Developing effective vaccines against Hib and non-typeable strains is crucial.

    Purpose of the Study:

    • To comparatively assess the immunobiological characteristics of three different antigenic preparations of Hib.
    • To evaluate the potential of a novel combination vaccine against Hib and non-typeable H. influenzae.

    Main Methods:

    • Assessed CPS Hib (cetavlon), HAP Hib, and a CPS Hib/LOS NTHi mixture (detoxified).
    • Studied effects on mouse spleen mononuclear leukocyte immunophenotype.
    • Analyzed serum cytokine profiles.

    Main Results:

    • The CPS Hib and detoxified LOS NTHi mixture exhibited low toxicity.
    • This combination demonstrated significant protective activity during Hib challenge.
    • It effectively activated innate immunity and initiated adaptive immune responses.

    Conclusions:

    • The developed preparation offers a promising strategy for protection against both capsular and acapsular H. influenzae strains.
    • This approach could lead to broader protection against diverse H. influenzae infections.
    Abstract

    More Related Videos

    Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes13:53

    Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

    Published on: December 19, 2020

    An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers06:34

    An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

    Published on: December 1, 2017

    Related Concept Videos

    Inhibitors Of Virion Release01:25

    Inhibitors Of Virion Release

    Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
    Antigens Involved in Adaptive Immunity01:26

    Antigens Involved in Adaptive Immunity

    An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
    Complete Antigens
    Complete antigens possess both immunogenicity and reactivity.
    Membrane Carbohydrates01:30

    Membrane Carbohydrates

    The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
    Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
    Immune Response Against Viral Pathogens01:29

    Immune Response Against Viral Pathogens

    The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
    NK Cells
    NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
    Immunoglobulin-like Cell Adhesion Molecules01:31

    Immunoglobulin-like Cell Adhesion Molecules

    Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
    Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...