Related Experiment Video
Updated: Jun 3, 2026
08:48
Peptide-derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants
Published on: December 16, 2016
Methods of gene transfer and analysis in higher plants
Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
Summary
Plant genetic engineering advances allow regeneration from cells and gene manipulation. Transgenic dicotyledons are common, with initial successes reported in monocotyledons like rye, maize, and rice.
Area of Science:
- Plant biotechnology
- Molecular biology
- Genetics
Background:
- Recent advancements in plant tissue culture and recombinant-DNA technology.
- Regeneration of diverse plant species from cultured cells is now feasible.
- Genetic information manipulation offers new avenues for crop improvement.
Purpose of the Study:
- To review the progress in plant genetic engineering.
- To highlight the development of transgenic plants.
- To discuss the successes and limitations in dicotyledons and monocotyledons.
Main Methods:
- Application of tissue culture techniques for plant regeneration.
- Utilizing recombinant-DNA technology for gene transfer.
- Analysis of gene integration and expression in transgenic plants.
Main Results:
- Successful regeneration of numerous transgenic dicotyledonous plants, especially in the Solanaceae family.
- Limited but significant success in generating transgenic monocotyledonous plants.
- Reported production of transgenic rye, maize, and rice plants.
Conclusions:
- Plant tissue culture and genetic engineering are powerful tools for plant science.
- Transgenic technology is well-established for dicotyledons.
- Further research is needed to expand success in monocotyledonous species.
Related Concept Videos
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
00:53Transgenic Organisms
Overview
01:59Plant Breeding and Biotechnology
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
02:19Export of Mitochondrial and Chloroplast Genes
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred irrespective...
02:18Types of Genetic Transfer Between Organisms
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
02:57Plant Tissue Culture
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.