Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2026

Swimming Exercise Protocol and Care Methods for Pregnant Rats
05:17

Swimming Exercise Protocol and Care Methods for Pregnant Rats

Published on: April 5, 2024

Mating of rats.

C H Phang1

  • 1Neuropeptide Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

Controlled matings produce one-cell fertilized eggs for transgenic rat production. Females undergo induced pseudopregnancy to serve as surrogate mothers for microinjected eggs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Introduction to the physiology and husbandry of the rat.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Vasectomy of the male rat.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Harvest of fertilized one-cell rat eggs.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Implantation of microinjected eggs.

Methods in molecular biology (Clifton, N.J.)·2011

Area of Science:

  • Reproductive biology
  • Genetics
  • Animal models

Background:

  • Transgenic rat production is crucial for research.
  • Efficient methods are needed to obtain viable embryos and surrogate mothers.

Purpose of the Study:

  • To outline the controlled conditions for obtaining fertilized eggs.
  • To describe the process of inducing pseudopregnancy in female rats for surrogacy.

Main Methods:

  • Controlled mating protocols to synchronize estrus and fertilization.
  • Surgical or non-surgical procedures to induce pseudopregnancy in recipient females.
  • Collection of one-cell stage fertilized eggs.

Main Results:

  • Successful procurement of a supply of one-cell fertilized rat eggs.

More Related Videos

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats
06:38

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Published on: October 13, 2018

Related Experiment Videos

Last Updated: Jun 3, 2026

Swimming Exercise Protocol and Care Methods for Pregnant Rats
05:17

Swimming Exercise Protocol and Care Methods for Pregnant Rats

Published on: April 5, 2024

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats
06:38

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Published on: October 13, 2018

  • Establishment of pseudopregnant female rats suitable for embryo transfer.

    • Conclusions:

    • Controlled matings and induced pseudopregnancy are essential steps for successful transgenic rat generation.
    • These foundational techniques ensure the availability of necessary biological materials for microinjection and subsequent development.