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Related Concept Videos

Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
PCR01:32

PCR

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Related Experiment Video

Updated: Jun 3, 2026

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
15:28

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Published on: September 3, 2009

Primer extension.

D R Smith1

  • 1Molecular Neurobiology Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

Primer extension is a molecular biology technique used to determine the size of RNA transcripts and map transcription start sites. This method involves extending a DNA probe along an RNA template, offering an alternative to S1 nuclease mapping.

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Last Updated: Jun 3, 2026

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
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Published on: September 3, 2009

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
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Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • S1 nuclease mapping is a technique that analyzes DNA:RNA hybrids by resecting them to the point of divergence.
  • Primer extension serves as a complementary method to S1 nuclease mapping in molecular biology analyses.

Purpose of the Study:

  • To describe the primer extension technique and its applications.
  • To highlight its utility in RNA transcript sizing and transcription initiation site mapping.

Main Methods:

  • Annealing a DNA probe to an RNA template.
  • Extending the DNA probe in a 3' to 5' direction along the RNA template until the start of the RNA molecule is reached.

Main Results:

  • Primer extension allows for the accurate determination of full-length RNA transcript sizes.
  • The technique effectively maps the initiation sites of transcription.

Conclusions:

  • Primer extension is a valuable technique for analyzing RNA transcripts.
  • It provides complementary information to S1 nuclease mapping, particularly for transcript size and transcription start site identification.