Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

105.6K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
105.6K
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

6.8K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
6.8K
SDS-PAGE01:27

SDS-PAGE

31.1K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
31.1K
Electrophoresis: Overview01:20

Electrophoresis: Overview

3.0K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
3.0K
Southern Blot02:57

Southern Blot

21.2K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
21.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Public health centres in contemporary Japan.

Public health·2009
Same author

Broadband ground-plane cloak.

Science (New York, N.Y.)·2009
Same author

A collective scattering system for measuring electron gyroscale fluctuations on the National Spherical Torus Experiment.

The Review of scientific instruments·2009
Same author

Optical source transformations.

Optics express·2008
Same author

Spatial resolution study and power calibration of the high-k scattering system on NSTX.

The Review of scientific instruments·2008
Same author

Running in new and worn shoes: a comparison of three types of cushioning footwear.

British journal of sports medicine·2008
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Nov 6, 2025

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
09:24

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Published on: June 14, 2016

26.4K

Agarose gel electrophoresis.

D R Smith1

  • 1Molecular Neurobiology Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

Agarose gel electrophoresis separates DNA fragments after restriction enzyme digestion. This technique utilizes a porous agarose matrix to visualize DNA, crucial for molecular biology applications.

More Related Videos

Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

780.7K
Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

34.3K

Related Experiment Videos

Last Updated: Nov 6, 2025

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
09:24

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

Published on: June 14, 2016

26.4K
Agarose Gel Electrophoresis for the Separation of DNA Fragments
07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

780.7K
Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

34.3K

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • Restriction enzyme digestion of DNA generates fragments requiring separation.
  • Agarose gel electrophoresis is a standard technique for separating DNA fragments.
  • Agarose, a seaweed-derived polymer, forms a gel matrix with pores whose size is concentration-dependent.

Purpose of the Study:

  • To describe the principles and methods of agarose gel electrophoresis.
  • To explain the preparation and use of agarose gels for DNA analysis.

Main Methods:

  • Agarose powder is melted in buffer and cooled to form a gel matrix.
  • Gel pore size is determined by agarose concentration (0.3-3%).
  • Submarine gel apparatus is commonly used, where the gel is submerged in electrophoresis buffer.

Main Results:

  • Agarose gels effectively separate DNA fragments ranging from 200 to 20,000 base pairs.
  • The concentration of agarose dictates the pore size and separation capability.
  • Loading buffer is used to ensure sample sinking into preformed wells.

Conclusions:

  • Agarose gel electrophoresis is an essential technique for DNA fragment separation and visualization.
  • The submarine gel system is the predominant apparatus for running agarose gels.
  • Proper gel preparation and sample loading are critical for successful DNA analysis.