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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
PCR01:32

PCR

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Related Experiment Video

Updated: Jun 3, 2026

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

Published on: October 8, 2019

Random primed labeling of DNA.

D R Smith1

  • 1Molecular Neurobiology Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

Random primed labeling is a DNA labeling technique that incorporates radioactive nucleotides. This method, based on Feinberg and Vogelstein, offers high specific activity for DNA probes.

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Last Updated: Jun 3, 2026

DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling
08:04

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Published on: October 8, 2019

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

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Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

Area of Science:

  • Molecular Biology
  • Biotechnology

Background:

  • Random primed labeling has largely replaced nick translation for DNA labeling.
  • It is based on the method developed by Feinberg and Vogelstein.

Purpose of the Study:

  • To describe the method of random primed labeling for DNA.
  • To highlight its advantages over older techniques.

Main Methods:

  • DNA denaturation by heat.
  • Annealing of random sequence oligonucleotides to DNA strands.
  • Extension of oligonucleotides by Klenow fragment polymerase using labeled nucleotides.

Main Results:

  • Achieves specific activities between 2 × 10^9 and 5 × 10^9 dpm/μg.
  • Produces uniformly labeled double-stranded DNA probes.
  • Applicable to any DNA template due to comprehensive oligonucleotide sequences.

Conclusions:

  • Random primed labeling is an efficient and versatile method for generating radioactive DNA probes.
  • The technique provides high specific activity and broad applicability.