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The amplification refractory mutation system.

¹Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR.

Methods in Molecular Medicine

|March 11, 2011

Summary

This summary is machine-generated.

Amplification Refractory Mutation System (ARMS) uses polymerase chain reaction (PCR) primers for allele-specific amplification. This method combines amplification and diagnostics for time-efficient genetic analysis.

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Area of Science:

Molecular Biology
Genetics

Background:

The Amplification Refractory Mutation System (ARMS) is a polymerase chain reaction (PCR) based amplification strategy.
ARMS is also known as allele-specific PCR or PCR amplification of specific alleles (PASA).

Purpose of the Study:

To describe the ARMS technique for discriminating single nucleotide differences in DNA templates.
To highlight the advantages and limitations of ARMS in genetic analysis.

Main Methods:

Designing PCR primers that are specific to a particular DNA allele.
Utilizing the primer's ability to remain refractory to amplification of other alleles differing by a single nucleotide.

Main Results:

ARMS allows for the amplification of a specific allele while preventing the amplification of others.
The presence of an amplified product directly indicates the presence of the targeted allele.

Conclusions:

ARMS offers a time-efficient method for routine genetic diagnosis by combining amplification and diagnostic steps.
The integrated nature of ARMS may reduce robustness compared to methods with separated amplification and analysis steps.