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Updated: Jun 3, 2026

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
Published on: June 14, 2024
Occurrence of leptospirosis among suspected cases in Chennai, Tamil Nadu
Ganesan Arumugam1, S Mini Jacob, D Anitha
1Department of Experimental Medicine, TN Dr. MGR Medical University, Chennai, Guindy, Tamil Nadu, India.
Objective:
To evaluate the serological profile of leptospirosis by microscopic agglutination test (MAT) and dark field microscopy (DFM) and to determine the serovar prevalence rate among patients with pyrexia of unknown origin.
Materials And Methods:
A total of 3830 blood samples were received from different hospitals and laboratories in and around Chennai. They were screened for leptospirosis by MAT and direct observation of live Leptospira by DFM.
Results:
A total of 748 (19.5%) Leptospira positive cases were identified; among these, 36.76% were Leptospira australis, 30% were Leptospira canicola, 14.57% were Leptospira autumnalis, 12% were Leptospira icterohaemorrhagiae, 4.68% were Leptospira patoc and 1.87% were Leptospira grippotyposa. Patients were in the age group of 1-86 years, with a median age of 43.5 years. 50% positive cases were in the age group of 10-35 years. Majority of the Leptospira infected cases were males (62.98%) than females (37.02%).
Conclusion:
Leptospirosis occurs in Chennai throughout the year although the number and positivity of cases increased during the monsoon season.
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