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Synthetic Antigen Controls for Immunohistochemistry
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Production of antisera to synthetic peptides

W J Gullick1

  • 1Protein Chemistry Laboratory, Institute of Cancer Research, Chester Beatty Laboratories, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 15, 2011
PubMed

The objective of immunization is to produce polyclonal or monoclonal antibodies that react with a chosen molecule. Immunization may be in vivo by injecting antigen into animals to produce antisera or to provide B-lymphocytes for immortalization as hybridomas, or alternatively, in vitro using splenocytes from untreated animals (1) for subsequent fusion with myeloma cells to produce monoclonal antibodies (MAbs).

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