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Updated: Jun 3, 2026

Immunoblot Analysis
16:01

Immunoblot Analysis

Published on: June 20, 2008

Use of dried milk for immunoblotting

R Jagus1, J W Pollard

  • 1Department of Microbiology, Biochemistry, and Molecular Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.

Methods in Molecular Biology (Clifton, N.J.)
|March 15, 2011
PubMed

Immunoprobing for specific peptides on solid supports such as nitrocellulose requires prior blocking of nonspecific protein binding sites. Many proteins, or protein mixtures, have been used for this, including bovine serum albumin, gelatin, and calf serum, but the cheapest and most efficient method makes use of dried milk protein.

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