Adaptive optics wide-field microscopy using direct wavefront sensing
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Updated: Jun 3, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
Published on: December 30, 2025
Oscar Azucena1, Justin Crest, Shaila Kotadia
1Jack Baskin School of Engineering, University of California, Santa Cruz, Santa Cruz, California 95064, USA. azucena@soe.ucsc.edu
Abstract:
We report a technique for measuring and correcting the wavefront aberrations introduced by a biological sample using a Shack-Hartmann wavefront sensor, a fluorescent reference source, and a deformable mirror. The reference source and sample fluorescence are at different wavelengths to separate wavefront measurement and sample imaging. The measurement and correction at one wavelength improves the resolving power at a different wavelength, enabling the structure of the sample to be resolved.

