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Aptamers: turning the spotlight on cells.

Dalia López-Colón1, Elizabeth Jiménez, Mingxu You

  • 1Department of Chemistry, University of Florida, Gainesville, FL, USA.

Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
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Summary

Aptamer probes, modified with dyes and nanomaterials, offer advanced cell imaging capabilities. These probes enable effective in vitro and in vivo imaging across multiple modalities for biomedical applications.

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Area of Science:

  • Biomedical Engineering
  • Molecular Imaging
  • Nanotechnology

Background:

  • Aptamers are nucleic acid or peptide molecules that bind to specific targets.
  • Cell imaging is crucial for understanding biological processes and disease.
  • Traditional imaging probes face limitations in specificity and targeting.

Purpose of the Study:

  • To review the development of aptamer probes for live cell imaging.
  • To discuss the modification of aptamers with imaging agents.
  • To highlight the applications of aptamer probes in various imaging techniques.

Main Methods:

  • Selection of aptamers against whole cells.
  • Conjugation of aptamers with fluorescent dyes, gold nanoparticles, quantum dots, and superparamagnetic iron oxide.
  • Evaluation of aptamer probes in vitro and in vivo imaging studies.

Main Results:

  • Aptamer probes demonstrate high specificity and affinity for target cells.
  • Modified aptamers successfully function as probes in optical imaging, MRI, CT, and PET.
  • In vitro and in vivo imaging of live cells using aptamer-based probes has been achieved.

Conclusions:

  • Aptamer probes represent a promising tool for advanced cell imaging.
  • The versatility of aptamer modification allows for diverse imaging applications.
  • Further development of aptamer probes will enhance biomedical diagnostics and therapeutics.