Preparation of Recombinant Plasmid DNA for DNA-Mediated Gene Transfer

Recombinant plasmid constructs are frequently employed in transfection experiments. With the availability of a wide spectrum of specialized and versatile eucaryotic cloning/expression vectors, investigators have been given powerful tools to expedite the elucidation of mechanisms governing gene expression, and to facilitate the identification of genes participating in diverse cellular processes (namely, metabolism, the immune response, differentiation and development, the repair of DNA damage, and malignant transformation).