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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 3, 2026

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
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Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Polybrene/DMSOAssisted Gene Transfer.

R Aubin1, M Weinfeld, M C Paterson

  • 1Molecular Genetics and Carcinogenesis Laboratory, W. W. Cross Cancer Institute, Edmonton, Alberta, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a novel two-stage gene transfer method using polybrene and dimethyl sulfoxide (DMSO) for efficient DNA delivery into mammalian cells. This technique enhances transfection rates while maintaining cell viability and clonogenicity.

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Last Updated: Jun 3, 2026

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Background:

  • Transfection of mammalian cells is often limited by low cell tolerance to gene transfer methods.
  • Synthetic polycations are explored for delivering exogenous DNA without compromising cell viability.
  • Polybrene is known to enhance retroviral infectivity by bridging viral particles and cell membranes.

Purpose of the Study:

  • To develop an efficient and simple gene transfer method for various mammalian cell types.
  • To improve DNA delivery efficiency while maintaining cell viability and clonogenicity.

Main Methods:

  • A two-stage gene transfer protocol was employed.
  • Stage 1: Polybrene facilitates binding of exogenous DNA to target cells.
  • Stage 2: Dimethyl sulfoxide (DMSO) permeabilizes cells for DNA uptake.

Main Results:

  • Achieved high efficiency in transfecting a variety of mammalian cell types.
  • Stable transfection frequencies ranged from 0.01% to 0.1% using nanogram quantities of DNA.
  • The method is simple to perform and preserves cell viability and clonogenicity.

Conclusions:

  • The described two-stage method using polybrene and DMSO is highly effective for gene transfer in mammalian cells.
  • This approach offers a viable alternative for transfecting cell types previously resistant to gene transfer.
  • The technique facilitates both stable integration and transient expression of foreign genes.