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Updated: Jun 3, 2026
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
[Prokaryotic expression and biological activity of recombinant human IL-17F/His protein]
Gao-qin Liu1, Hong-ya Wu, Long-biao Li
1Department of Ophthalmology, First Affiliated Hospital, Soochow University, Suzhou 215007, China. liugaoqin2006@sina.com
Aim:
To Prepare recombinant human IL-17F/His protein and investigate its biological activity in vitro.
Methods:
The gene region of human IL-17F was cloned by RT-PCR. After identification by sequencing, the hIL-17F gene encoding function domain was cloned into expression plasmid PQE3.0 and transfected into E.coli M15. By the induction of Isopropyl-β-D-Thiogalacto-Pyranoside(IPTG), recombinant IL-17F/His protein was effectively expressed in E.coli M15. The recombinant protein was identified by Western blot.
Results:
After renaturation and purification by HiTrap(TM); affinity column, the recombinant protein can up-regulate macrophages to secret TNF-α, IL-6 and other relative cytokines. It also promoted proliferation of HeLa cells in vitro.
Conclusion:
hIL-17F/His recombinant protein is of high biological activity, which can be used to make further study of its special characteristic.
