Secondary metabolites from Asperula lutea subsp. rigidula

From the aerial parts of Asperula lutea subsp. rigidula (Halacsy) Ehrend., an endemic plant of south-eastern Greece, nine known compounds, beta-sitosterol, geniposidic acid, deacetyl-asperuloside, scandoside, chlorogenic acid, quercetin, hyperin, isoquercitrin and rutin, were isolated. From the analysis of a non polar fraction of the methanolic extract by means of GC and GC-MS, eighteen compounds were identified, mainly esters of fatty acids. The major components were the ethyl esters ofpalmitic acid (32.7%), linolenic acid (20.1%) and linoleic acid (10.5%).