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Updated: Jun 3, 2026

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
Published on: September 17, 2016
Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis
Kevin Ashelford1, Maria E Eriksson, Christopher M Allen
1School of Biological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK.
Abstract:
Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.
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