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A coumarin-based fluorescent probe for Hg2+ and Ag+ with an N'-acetylthioureido group as a fluorescence switch

Koji Tsukamoto1, Yuka Shinohara, Shogo Iwasaki

  • 1School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Kobe, Hyogo 650-8530, Japan. kotukamoto@huhs.ac.jp

Chemical Communications (Cambridge, England)
|March 25, 2011
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...