Investigations on the PGM (phosphoglucomutase) polymorphism by isoelectric focusing in Drosophila melanogaster

Pgm allele frequencies of 383 individuals were determined in a sample of Drosophila melanogaster from three laboratory Sardinian populations, using the techniques of standard electrophoresis, heat denaturation, and isoelectric focusing. The analysis of the progeny obtained from informative crosses showed that the isoelectric focusing patterns segregate in a Mendelian way. The Pgm1.00 and Pgm0.70 electrophoretic alleles displayed different isoelectric points, whereas the Pgm1.00,tr and Pgm1.00,ts isoelectrophoretic alleles could not be differentiated when tested by isoelectric focusing. Moreover, the Pgm0.70,ts allele was split into two classes, with isoelectric points of pH 6.4 and pH 6.6.