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Related Concept Videos

IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single stretching vibration...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

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Published on: February 4, 2017

Quantum-cascade laser-based vibrational circular dichroism.

Steffen Lüdeke1, Marcel Pfeifer, Peer Fischer

  • 1Institute for Pharmaceutical Sciences, University of Freiburg, Albertstr. 25, 79104 Freiburg, Germany.

Journal of the American Chemical Society
|March 31, 2011
PubMed
Summary
This summary is machine-generated.

Tunable quantum-cascade lasers (QCLs) offer powerful new capabilities for vibrational circular dichroism (VCD) spectroscopy. This bright light source enables VCD studies in challenging, strongly absorbing solvents like water.

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Area of Science:

  • Spectroscopy
  • Physical Chemistry
  • Quantum Optics

Background:

  • Vibrational circular dichroism (VCD) spectroscopy is a powerful technique for determining molecular chirality.
  • Traditional VCD measurements are limited by the low power of infrared light sources, especially in strongly absorbing solvents.
  • Strongly absorbing solvents like water pose significant challenges for VCD analysis due to high signal attenuation.

Purpose of the Study:

  • To investigate the potential of tunable external-cavity quantum-cascade lasers (QCLs) as a novel light source for VCD spectroscopy.
  • To demonstrate the enhanced brightness and applicability of QCLs for VCD measurements in challenging media.
  • To showcase the utility of QCL-based VCD for analyzing compounds in aqueous solutions.

Main Methods:

  • Recording vibrational circular dichroism (VCD) spectra using a tunable external-cavity quantum-cascade laser (QCL).
  • Performing infrared (IR) absorption measurements with the same QCL system.
  • Utilizing a range of compounds, including proline in water, to demonstrate the method's effectiveness.

Main Results:

  • QCLs provide significantly higher power output compared to standard infrared thermal light sources.
  • The enhanced brightness of QCLs enables VCD and IR absorption measurements in strongly absorbing solvents.
  • Successful VCD and IR absorption spectra were obtained for proline in water, demonstrating feasibility.

Conclusions:

  • Tunable external-cavity quantum-cascade lasers represent a promising advancement for VCD spectroscopy.
  • The high power and tunability of QCLs overcome limitations associated with traditional light sources.
  • QCL-based VCD spectroscopy opens new avenues for chiral analysis in diverse and challenging sample environments, including aqueous solutions.