Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectrally encoded flow cytometry using few-mode fiber collection.

Biomedical optics express·2025
Same author

Measuring the Acoustic Reflex through the Tympanic Membrane.

Audiology & neuro-otology·2024
Same author

Pinhole shifting for reducing speckle contrast in reflectance confocal microscopy.

Optics letters·2023
Same author

In vivo optical mapping of the tympanic membrane impulse response.

Hearing research·2023
Same author

Measuring the red blood cell shape in capillary flow using spectrally encoded flow cytometry.

Biomedical optics express·2022
Same author

Optimization study of plasmonic cell fusion.

Scientific reports·2022
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 3, 2026

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence
10:28

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

Spectrally encoded spectral imaging.

Avraham Abramov1, Limor Minai, Dvir Yelin

  • 1Department of Biomedical Engineering, Technion-Israel Institute of Technology, Technion City, Haifa, Israel.

Optics Express
|April 1, 2011
PubMed
Summary
This summary is machine-generated.

A new fiber-based spectrally encoded spectral imaging (SESI) technique captures full spectra from specimens. This method offers potential for improved signal-to-noise ratio and minimally invasive endoscopic applications.

More Related Videos

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
06:28

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Published on: October 7, 2014

Related Experiment Videos

Last Updated: Jun 3, 2026

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence
10:28

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
06:28

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

Published on: October 7, 2014

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Spectroscopy

Background:

  • Spectral imaging is crucial for scientific and technological applications, particularly in biomedicine for specimen analysis and clinical diagnosis.
  • Challenges in biomedical spectral imaging include low damage thresholds and strict time constraints.
  • Existing techniques may not fully capture spectral data from each sample point efficiently.

Purpose of the Study:

  • To introduce a novel fiber-based technique, spectrally encoded spectral imaging (SESI), for enhanced spectral data acquisition.
  • To demonstrate the capability of SESI in capturing spectral data cubes from various samples.
  • To theoretically evaluate the signal-to-noise ratio advantages of SESI.

Main Methods:

  • Development of a fiber-based system utilizing spectrally encoded lines and lateral scanning.
  • Implementation of a miniaturized grating-lens configuration for endoscopic compatibility.
  • Acquisition of spectral data cubes from a color print and a green leaf.

Main Results:

  • Successful demonstration of SESI in capturing detailed spectral data from diverse samples.
  • Theoretical discussion highlighting potential improvements in signal-to-noise ratio compared to conventional methods.
  • Feasibility of endoscopic application using a compact SESI system.

Conclusions:

  • SESI provides a powerful new approach for spectral imaging, overcoming some limitations of existing techniques.
  • The technique shows promise for minimally invasive spectral and color imaging in remote biological locations.
  • SESI has potential for advancing biomedical diagnostics and scientific research through enhanced spectral data acquisition.