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Related Experiment Video

Updated: Apr 3, 2026

The bm12 Inducible Model of Systemic Lupus Erythematosus SLE in C57BL/6 Mice

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Published on: November 1, 2015

Biosprayed spleen cells integrate and function in mouse models.

Natalie A Carter¹, Suwan N Jayasinghe, Claudia Mauri

¹Centre for Rheumatology Research, Division of Medicine, University College London, 46 Cleveland Street, London, W1T 4JF, UK.

|April 1, 2011

Summary

This summary is machine-generated.

Bio-electrospraying (BES) and aerodynamically assisted bio-jetting (AABJ) are safe cell handling techniques. In vivo studies confirm sprayed cells maintain biological functions, showing potential for advanced cell-based applications.

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Area of Science:

Biotechnology
Cell Biology
Immunology

Background:

Non-contact direct cell handling methods like bio-electrospraying (BES) and aerodynamically assisted bio-jetting (AABJ) require validation for preserving cell integrity.
Previous in vitro studies indicated these biospray techniques are inert for accurate cell distribution.

Purpose of the Study:

To validate the in vivo inertness of BES and AABJ techniques.
To determine if splenic cells are functionally or phenotypically altered by these spray methods.

Main Methods:

Utilizing an adoptive transfer mouse model (C57/BL6 mice).
Comparing the in vivo behavior, survival, proliferation, and migration of sprayed splenic cells against unmanipulated controls.

Main Results:

Sprayed splenic cells exhibited indistinguishable in vivo behavior compared to unmanipulated cells.
Cells processed via BES and AABJ demonstrated similar survival and proliferation rates upon antigen activation.
In vivo migration characteristics of sprayed cells were identical to those of unmanipulated cells.

Conclusions:

BES and AABJ are confirmed as inert biospray techniques that do not alter splenic cell function or phenotype in vivo.
These biotechniques show significant potential for applications in 3D cultures, cell interaction monitoring, and disease modeling.