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TaSRG, a wheat transcription factor, significantly affects salt tolerance in transgenic rice and Arabidopsis
Xiaoliang He1, Xiaona Hou, Yinzhu Shen
1College of Life Science of Hebei Normal University, No. 113 Yuhua East Road, Shijiazhuang, Hebei, China.
Abstract:
Based on our microarray-analysis results of the salt tolerant wheat mutant, RH8706-49, an unknown salt-induced gene containing a conserved DUF662 domain was identified and cloned; we named this gene TaSRG (Triticum aestivum Salt Response Gene). Real-time quantitative PCR analyses showed that the expression of this gene was affected by salt, drought, cold, ABA and other stress conditions. The overexpression of TaSRG in wild-type Arabidopsis resulted in increased salt tolerance compared with wild-type plants. In addition, subcellular localisation revealed that TaSRG encodes a protein that was found mainly in the nucleus. Our results suggest that wheat TaSRG might encode a transcription factor that could be utilised for enhancing stress tolerance in a wide range of plants.
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