Related Experiment Video
Updated: Jun 3, 2026

Murine Short Axis Ventricular Heart Slices for Electrophysiological Studies
Published on: June 4, 2017
Contribution of Nav1.1 to cellular synchronization and automaticity in spontaneous beating cultured neonatal rat
1Division of Physiome, Department of Physiology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan. masumiya@hyo-med.ac.jp
Abstract:
Two factors of cell coupling influence cellular synchronization and automaticity: gap junction coupling and ion channels activity. However, the role of Na(+) channel isoforms underlying cell-to-cell interaction and cellular automaticity is not well understood. To address these questions, we studied mRNA expression of Na(+) channel isoforms and the effects of TTX on spontaneously beating cultured ventricle cells. Using RT-PCR technique we demonstrated the presence of Na(v)1.1 and Na(v)1.5 channels. The reduction of Na(v)1.1 channel activity disturbed cell-to-cell interaction and changed beating rates. Thus, Na(v)1.1 channel is involved in cellular synchronization and automaticity.
More Related Videos
09:20Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice
Published on: July 5, 2021
06:22Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
Published on: November 3, 2020
Related Concept Videos
Mechanism of Cardiac Arrhythmias
Electrophysiology of Normal Cardiac Rhythm