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Published on: May 4, 2018
[Phenotypic characteristics of Pseudomonas putida isolated from different environments]
1Wielkopolskie Centrum Onkologii im. Marii Skłodowskiej Curie.
Abstract:
Various typing methods have been suggested to differentiate isolates of P. putida species with the aim of developing epidemiological tools. 34 P putida strains were isolated from the samples of crude oil and oil derivates contaminated soil (n=27), biopreparates used for biodegradation of soil contamination (n=3) and hospital materials (n=4). The biochemical typing was assessed using ID32GN tests (bio-Merieux). The strains were grouped into 11 biotypes. Antibiotic-containing discs were used for routine antibiogramsby disc diffusion assay. The strains were most resistant to cefoperazone and ticarcillin (29.4% and 26.5% of strains, respectively). The intracellular esterases of P. putida were separated by polyacrylamide gel electrophoresis and stained with Fast Blue using alpha-naphthyl acetate, beta-naphthyl propionate and indoxyl acetate as substrates. On the basis of the indicators dye migration the electrophoresis process was observed. By calculating the RF (retention factor) the distribution of stripes was obtained with great accuracy. The use of biochemical tests, sensitivity tests and zymotyping provides the possibility to inter- and intraspecies differentiation of P. putida.
Insights
This study differentiated Pseudomonas putida strains using biochemical tests, antibiotic resistance, and zymotyping. These methods effectively distinguish between P. putida strains for epidemiological applications.
Area of Science:
- Microbiology
- Molecular Biology
Context:
- Pseudomonas putida (P. putida) is an important bacterium found in various environments, including contaminated soil and clinical settings.
- Accurate differentiation of P. putida strains is crucial for epidemiological studies and understanding their ecological roles.
Purpose:
- To evaluate the effectiveness of biochemical tests, antibiotic susceptibility testing, and zymotyping for differentiating P. putida isolates.
- To establish reliable typing methods for epidemiological surveillance of P. putida.
Summary:
- Thirty-four P. putida strains were isolated from soil, biopreparates, and hospital materials.
- Biochemical typing (ID32GN) identified 11 biotypes.
- Antibiotic resistance patterns showed high resistance to cefoperazone and ticarcillin.
- Polyacrylamide gel electrophoresis (PAGE) of intracellular esterases (zymotyping) provided accurate strain differentiation based on retention factors (RF).
Impact:
- Combined use of biochemical tests, antibiotic sensitivity, and zymotyping offers a robust approach for inter- and intraspecies differentiation of P. putida.
- These methods can serve as valuable epidemiological tools for tracking P. putida strains.
