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Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase
Published on: July 23, 2009
Gene expression changes: five years after creation of elastase-induced aneurysms.
Ramanathan Kadirvel1, Yong-Hong Ding, Daying Dai
1Department of Radiology, Mayo Clinic, Rochester, MN 55905, USA.
Journal of Vascular and Interventional Radiology : JVIR
|April 13, 2011
Summary
This study investigated gene expression in chronic saccular aneurysms in rabbits. Key differences in immune, growth, and structural genes offer insights into aneurysm development and potential therapeutic targets.
Area of Science:
- Vascular biology
- Genomics
- Pathophysiology
Background:
- Intracranial saccular aneurysms involve arterial wall remodeling.
- The growth and rupture mechanisms of aneurysms remain unclear.
Purpose of the Study:
- To investigate gene expression patterns in chronic elastase-induced saccular aneurysms.
- To compare gene expression in aneurysmal tissue with control arteries 5 years post-induction.
Main Methods:
- Elastase-induced saccular aneurysms were created in rabbits and followed for 5 years.
- Gene expression analysis was performed using human gene microarrays on aneurysm and control carotid artery tissues.
- Real-time polymerase chain reaction (RT-PCR) validated microarray findings.
Main Results:
- 0.4% of analyzed genes (53 out of 13,353) showed differential expression in aneurysms.
- Differentially expressed genes included those involved in immunoregulatory, growth factor, cell adhesion, and structural pathways.
- RT-PCR confirmed the differential gene expression observed in microarrays.
Conclusions:
- Chronic saccular aneurysms exhibit significant modulation of various biochemical and cellular functions.
- These findings provide molecular insights into the pathophysiology of saccular aneurysms.
