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Published on: December 30, 2016
Crystallization and preliminary X-ray diffraction analysis of cyclic imide hydrolase (CIH) from Pseudomonas putida
Zheng Fan1, Jianxun Qi, Yawei Shi
1Core Facility Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China. fanzh@im.ac.cn
Abstract:
A recombinant form of cyclic imide hydrolase from Pseudomonas putida YZ-26 has been crystallized by the hanging-drop method. X-ray diffraction data were collected to 1.9 Å resolution. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 111.91, b = 176.04, c = 176.06 Å. Assuming the presence of four molecules in the asymmetric unit gives a V(M) value of 3.10 Å(3) Da(-1) and a solvent content of 60.31%.
