Related Experiment Video
Updated: Jun 2, 2026
10:16
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
Published on: January 20, 2023
Cell sorting by endocytotic capacity in a microfluidic magnetophoresis device
Damien Robert1, Nicole Pamme, Hélène Conjeaud
1Laboratoire Matière et Systèmes Complexes, UMR CNRS et Université Paris Diderot, France.
Lab on a Chip
|April 23, 2011
Summary
This study demonstrates on-chip free-flow magnetophoresis for sorting magnetically labeled monocytes and macrophages. The method effectively separates cells based on their iron loading, showcasing potential for cell analysis and treatment applications.
Area of Science:
- Biotechnology
- Cell Biology
- Nanotechnology
Background:
- Magnetically labeled cells are crucial for in vitro and in vivo applications.
- Cell sorting based on magnetite loading is essential for these procedures.
- Endocytotic capacity influences nanoparticle internalization in cells.
Purpose of the Study:
- To investigate the sorting of monocytes and macrophages based on their differential nanoparticle uptake.
- To evaluate the efficacy of on-chip free-flow magnetophoresis for separating cells with varying iron loading.
- To establish a method for studying endocytotic capacity and cell selection.
Main Methods:
- Utilized on-chip free-flow magnetophoresis for cell sorting.
- Quantified iron loading in monocytes (1-4 pg/cell) and macrophages (4-60 pg/cell).
- Separated cell mixtures achieving high purity and efficacy.
Main Results:
- Macrophages were sorted into five subpopulations based on narrow iron loading distributions.
- Monocyte-macrophage mixtures were successfully sorted with >88% purity and >60% efficacy.
- Achieved high throughput ranging from 10 to 100 cells per second.
Conclusions:
- On-chip free-flow magnetophoresis is effective for sorting cells with similar iron loading.
- The method provides a viable tool for studying endocytotic capacity.
- This technique enables cell sorting and selection based on endocytotic functionality.
More Related Videos
Related Concept Videos
01:20Overview Of Cell Separation And Isolation
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
01:05Centrifugation
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...