Related Experiment Video
Updated: Jun 2, 2026

07:20
Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
Published on: August 31, 2018
Development of soybean aphid genomic SSR markers using next generation sequencing
Tae-Hwan Jun1, Andrew P Michel, M A Rouf Mian
1Department of Entomology, The Ohio State University - Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA.
Genome
|May 3, 2011
Summary
Researchers developed new molecular markers for soybean aphid (Aphis glycines) using next-generation sequencing. These simple sequence repeat markers are the first of their kind and will aid in studying aphid genetics and diversity.
Area of Science:
- Genomics
- Molecular Biology
- Entomology
Background:
- Soybean aphid (Aphis glycines) is a major pest of soybean (Glycine max) in North America.
- The emergence of aphid biotypes resistant to soybean cultivars necessitates genetic studies.
- Current genetic resources and molecular markers for soybean aphid are limited.
Purpose of the Study:
- To develop and characterize genomic simple sequence repeat (SSR) markers from soybean aphid sequences.
- To evaluate the utility of these SSRs for genetic diversity and relationship analyses in A. glycines.
Main Methods:
- Genomic sequences of A. glycines were generated using Illumina GAII next-generation sequencing.
- 128 SSR primer pairs were designed from these sequences.
- Polymorphism screening and PCR-based differentiation were performed on aphid samples.
Main Results:
- 120 out of 128 primer pairs successfully amplified SSR alleles.
- 24 SSR loci exhibited polymorphism among three aphid populations.
- Polymorphic SSRs differentiated 24 soybean aphid samples from different geographic locations.
Conclusions:
- Developed SSR markers are the first PCR-based, codominant markers for A. glycines.
- These markers are valuable for genetic differentiation and population studies of the soybean aphid.
- The markers will facilitate research into soybean aphid biotypes and pest management strategies.

