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Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Long-patch Base Excision Repair

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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Related Experiment Video

Updated: Jun 2, 2026

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

Pause locally, splice globally.

Fernando Carrillo Oesterreich¹, Nicole Bieberstein, Karla M Neugebauer

¹Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.

Trends in Cell Biology

|May 3, 2011

Summary

This summary is machine-generated.

Transcription and splicing are coupled processes in eukaryotes. RNA polymerase II elongation rates and chromatin structure influence how introns are removed from pre-mRNA, impacting gene expression.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Last Updated: Jun 2, 2026

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

Molecular Biology
Genetics
Gene Regulation

Background:

Eukaryotic protein-coding genes contain introns that must be removed via pre-messenger RNA (pre-mRNA) splicing.
Splicing is a critical step in gene expression, occurring concurrently with transcription by RNA polymerase II.
The precise mechanisms linking transcription and splicing remain an active area of research.

Purpose of the Study:

To review recent evidence demonstrating the functional coupling between transcription and splicing.
To explore the impact of RNA polymerase II elongation rates on splicing regulation.
To discuss the role of chromatin in mediating the interplay between transcription and splicing.

Main Methods:

Review of recent scientific literature and experimental findings.
Analysis of molecular mechanisms governing RNA polymerase II (RNAP II) elongation.
Integration of concepts from chromatin biology and splicing regulation.

Main Results:

Evidence supports a functional link between transcription elongation and splicing outcomes.
Transcriptional pausing and local elongation rates directly influence splicing decisions.
Chromatin features, including nucleosome positioning and histone modifications, modulate transcription-elongation and splicing factor recruitment.

Conclusions:

Transcription and splicing are dynamically coupled processes, not independent events.
RNA polymerase II dynamics are central to regulating splicing fidelity and efficiency.
Chromatin environment plays a crucial role in coordinating transcription and splicing.