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Updated: Jun 2, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
Published on: February 27, 2026
[Functional haemostatic tests are fast and reliable by transfusion demanding bleeding]
Sulman Rafiq1, Daniel A Steinbrüchel, Pär I Johansson
1Thoraxkirurgisk Afdeling, Rigshospitalet, København K. sulman_raf@hotmail.com
Viscoelastic haemostatic tests (VHT), including thrombelastography (TEG) and rotational thrombelastometry (ROTEM), offer rapid, point-of-care assessment of blood clotting. These assays enable quicker, more targeted interventions compared to traditional coagulation tests.
Area of Science:
- Coagulation Science
- Haemostasis Research
- Point-of-Care Diagnostics
Context:
- Viscoelastic haemostatic tests (VHT) such as thrombelastography (TEG) and rotational thrombelastometry (ROTEM) are cell-based whole blood assays.
- These tests evaluate the viscoelastic properties of blood coagulation.
- Understanding these properties is crucial for managing bleeding disorders and surgical haemostasis.
Purpose:
- To review the methodology and interpretation of TEG and ROTEM results.
- To discuss current clinical applications and limitations of VHT.
- To provide a comprehensive overview of viscoelastic haemostatic testing.
Summary:
- TEG and ROTEM assess coagulation by measuring viscoelastic changes in whole blood.
- The review details the techniques, result interpretation, and clinical uses of these point-of-care assays.
- VHT provide a rapid and comprehensive view of haemostatic function.
Impact:
- VHT are inexpensive, point-of-care tools for assessing haemostatic function.
- They facilitate faster and more focused interventions than traditional coagulation tests.
- This leads to improved patient management in critical care and surgical settings.
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