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Updated: Jun 2, 2026
Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases
Published on: May 17, 2019
Identification of differentially expressed genes in breast cancer
A Burger1, H Li, A Panayiotakis
1UNIV TORONTO,DEPT PATHOL,FAC MED,TORONTO,ON,CANADA. UNIV TORONTO,FAC DENT,TORONTO,ON,CANADA. UNIV TORONTO,WOMENS COLL HOSP,TORONTO,ON,CANADA. UNIV BRADFORD,CLIN ONCOL UNIT,BRADFORD BD7 1DP,W YORKSHIRE,ENGLAND. MEM SLOAN KETTERING CANC CTR,NEW YORK,NY. NCI,MOLEC ONCOL LAB,FREDERICK,MD.
Abstract:
A number of genes differentially expressed in breast cancer were isolated using a subtractive cloning technique. DNA sequence analysis and GenBank searches of T4F10, T2H7, and T2E5 cDNA clones found them to be identical with E2A, MSS1, and SEC13R genes. Their expression in a variety of primary breast tumor and cancer cell lines was compared with c-ERB-B2 and pS2 by Northern blot analysis. In breast cancer cell lines, the genes that we isolated are more frequently expressed than the previously described c-ERB-B2 and pS2.
Insights
Researchers identified novel genes, E2A, MSS1, and SEC13R, differentially expressed in breast cancer. These genes show higher expression in breast cancer cell lines compared to known markers c-ERB-B2 and pS2.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Breast cancer is a heterogeneous disease with complex genetic underpinnings.
- Identifying novel genes involved in breast cancer progression is crucial for developing targeted therapies.
- Established biomarkers like c-ERB-B2 and pS2 are important but do not capture the full spectrum of gene expression changes.
Purpose of the Study:
- To isolate and identify novel genes differentially expressed in breast cancer.
- To compare the expression patterns of newly identified genes with known breast cancer markers.
Main Methods:
- Subtractive cloning technique was employed to isolate differentially expressed genes.
- DNA sequence analysis and GenBank searches were performed on isolated cDNA clones (T4F10, T2H7, T2E5).
- Northern blot analysis was used to compare gene expression in breast tumor and cell line samples.
Main Results:
- Three cDNA clones, T4F10, T2H7, and T2E5, were identified as the E2A, MSS1, and SEC13R genes, respectively.
- The expression of E2A, MSS1, and SEC13R genes was analyzed in various primary breast tumors and cancer cell lines.
- These newly identified genes demonstrated more frequent expression in breast cancer cell lines compared to the established markers c-ERB-B2 and pS2.
Conclusions:
- E2A, MSS1, and SEC13R are novel genes significantly expressed in breast cancer.
- These genes represent potential new biomarkers or therapeutic targets for breast cancer.
- Further research is warranted to elucidate the functional role of these genes in breast cancer pathogenesis.
