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Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
Published on: August 14, 2018
CD44 with variant exons 8-10 in colorectal tumors
T Goi1, A Yamaguchi, K Takeuchi
1NAGASAKI UNIV,SCH MED,DEPT ONCOL,NAGASAKI 852,JAPAN. FUKUI MED SCH,DEPT SURG 1,FUKUI 91011,JAPAN. MIE UNIV,SCH MED,DEPT INTERNAL MED 2,TSU,MIE 514,JAPAN.
International Journal of Oncology
|May 6, 2011
Summary
A novel CD44 variant (CD44v8-10) is expressed in colorectal and gastric cancers. Its detection correlates with metastasis, suggesting its potential as a biomarker for predicting colorectal cancer spread.
Area of Science:
- Oncology
- Molecular Biology
- Immunology
Background:
- CD44 is a cell surface glycoprotein involved in cell adhesion and signaling.
- Aberrant expression of CD44 variants is implicated in cancer progression.
- Specific CD44 variant exons (v8-10) have been investigated for their role in cancer.
Purpose of the Study:
- To generate a monoclonal antibody (mAb) reactive with the CD44 variant exon (v) 8-10 product.
- To investigate the expression of CD44v8-10 in colorectal and gastric cancer cell lines and tissues.
- To evaluate the correlation between CD44v8-10 expression and clinicopathological features, particularly metastasis.
Main Methods:
- Generation of a monoclonal antibody (mAb) 44-1V against a fusion protein of CD44 variant exons (v) 8-10.
- Western immunoblotting and reverse transcriptase-polymerase chain reaction/Southern blot to detect CD44v8-10 expression in cell lines.
- Immunohistochemical analysis of 179 pairs of colorectal cancer and normal tissues.
Main Results:
- A 130 kDa component containing CD44v8-10 products was specifically expressed in colorectal and gastric cancer cell lines.
- Approximately 47% of colorectal cancers examined expressed CD44v8-10.
- CD44v8-10 expression significantly correlated with hematogenous and lymph node metastasis in colorectal cancer patients.
Conclusions:
- CD44 variant exon (v) 8-10 is specifically expressed in colorectal and gastric cancers.
- The expression of CD44v8-10 is associated with increased metastatic potential in colorectal cancer.
- CD44v8-10 may serve as a valuable biomarker for predicting metastasis in colorectal cancer.
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