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The ammonium sulfate activation of phosphorylase b
D D Leonidas1, N G Oikonomakos, A C Papageorgiou
1Biological Research Center, National Hellenic Research Foundation, Athens, Greece.
FEBS Letters
|February 12, 1990
Abstract:
The ammonium sulfate activation of phosphorylase b has been studied. Ammonium sulfate, when present in high concentrations, induces properties of phosphorylase a in phosphorylase b, such as an enhanced affinity for AMP, a reversal of the glucose-6-P inhibition and enzyme tetramerization. The data are consistent with the interpretation that sulfates bind to the Ser-14 site and the sulfate-protein interactions at this site are responsible for activation of phosphorylase b.