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Updated: Jun 2, 2026
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
Published on: March 5, 2019
[Phenotypic analysis of BCG-specific effector memory CD4+ T cells in PBMCs by eight-color flow cytometry]
Li Li1, Xiao-ying Fu, Chang-you Wu
1Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Guangzhou 510080, China. ly_li@yahoo.cn
Aim:
To evaluate cytokine production and subsets in PBMCs from PPD+ normal donors after stimulation with BCG.
Methods:
PBMCs were isolated from PPD+ normal individuals, and cytokine production and BCG-specific T cell subsets were assessed by eight-color flow cytometry.
Results:
Following stimulation with BCG, CD4+ but not CD8+ T cells expressed IFN-γ, IL-2 and TNF-α. Phenotypic analysis indicated that cytokine-producing cells were CD4+CD45RO+CD62L-CD27- and CD4+CD45RO+CD62L-CD27+.
Conclusion:
BCG predominantly induced CD4+ T cells to produce cytokines following stimulation with BCG. Further analysis indicated that these cells are CD4+CD45RO+CD62L- effector memory cells, suggesting that these cells probably played essential role in preventing TB infection.
