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Updated: Jun 2, 2026
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Published on: October 20, 2021
[The study of CD4+ and CD8+ T subsets in chronic hepatitis B patients]
Xue-hua Sun1, Qiao-li Liu, Man Li
1Shuguang Hospital Affliated to Shanghai University of TCM, Cellular Immunity Laboratory of State Administration of TCM of China, Shanghai 200021, China. susan_sxh@sina.com
Insights
Certain T cell subsets, including CD4+CD25+, CD4+CD95+, and CD8+CD28-, are elevated in chronic hepatitis B (CHB) patients. These changes may contribute to immune tolerance in chronic HBV infection.
Area of Science:
- Immunology
- Hepatology
- Virology
Context:
- Chronic hepatitis B (CHB) is a significant global health concern.
- Understanding T cell subset dynamics is crucial for managing CHB.
- Immune responses play a key role in HBV infection outcomes.
Purpose:
- To investigate differences in CD4+ and CD8+ T cell subsets among CHB patients with varying infection statuses.
- To analyze the correlation between T cell subsets and viral markers (HBV DNA, HBeAg).
Summary:
- Flow cytometry revealed distinct T cell subset percentages in CHB patients compared to healthy controls.
- Elevated CD4+CD25+, CD4+CD95+, and CD8+CD28- subsets were observed in CHB patients.
- Correlations were found between specific T cell subsets and HBV DNA levels or HBeAg status.
Impact:
- Findings suggest specific T cell subsets may be involved in immune tolerance in chronic HBV infection.
- This research provides insights into the immunological mechanisms underlying CHB.
- Further research could explore therapeutic strategies targeting these T cell subsets.
Aim:
To investigate the difference of CD4+ and CD8+ T subsets in different infection status of chronic hepatitis B (CHB) patients.
Methods:
13 Healthy people were chosen as control group. According to infection status, 78 CHB patients were sorted into HBeAg(+) and normal liver function group, HBeAg(+) and abnormal liver function group, and HBeAg(-) group. The percentages of some subsets of CD4+T and CD8+T cells in CHB patients and healthy people were examined by Flow cytometer, and the correlations between the subsets of T cells and the HBVDNA levels and HBeAg were analyzed.
Results:
As compared with control group, the percentages of CD4+CD25+/CD4+ and CD4+CD95+/CD4+ were increased (P<0.01, P<0.05) in HBeAg(+) and normal liver function group, and the percentages of CD8+CD28-/CD8+ in three patients groups were increased (P<0.01). As compared with the HBeAg(+) and normal liver function group, the percentages of CD4+CD25+/CD4+ in HBeAg(+) and abnormal liver function group, and HBeAg(-) group were decreased (P<0.01), and the percentages of CD8+CD95+/CD8+ in HBeAg(-) group and the percentages of CD8+CD95+/CD8 in HBeAg(+) and abnormal liver function group were decreased (P<0.05, P<0.01). Moreover, there were positive correlations between CD4+CD25+/CD4+, CD4+CD95+/CD4+ and the HBVDNA levels(P<0.01); there were inverse correlations between CD4+CD28+/CD4+ and the HBVDNA levels and HBeAg (P<0.05, P<0.01); there were positive correlations between CD8+CD28-/CD8+ and HBVDNA and HBeAg (P<0.01).
Conclusion:
Some T cell Subsets, including CD4+CD25+, CD4+CD95+ , and CD8+CD28-, are increased in CHB patients, which maybe play some roles in the immune tolerance of chronic HBV infection.
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