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Updated: Jun 2, 2026

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
Published on: September 3, 2012
Central carbon metabolism in Mycobacterium tuberculosis: an unexpected frontier
Kyu Y Rhee1, Luiz Pedro Sorio de Carvalho, Ruslana Bryk
1Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA. kyr9001@med.cornell.edu
Abstract:
Recent advances in liquid chromatography and mass spectrometry have enabled the highly parallel, quantitative measurement of metabolites within a cell and the ability to trace their biochemical fates. In Mycobacterium tuberculosis (Mtb), these advances have highlighted major gaps in our understanding of central carbon metabolism (CCM) that have prompted fresh interpretations of the composition and structure of its metabolic pathways and the phenotypes of Mtb strains in which CCM genes have been deleted. High-throughput screens have demonstrated that small chemical compounds can selectively inhibit some enzymes of Mtb's CCM while sparing homologs in the host. Mtb's CCM has thus emerged as a frontier for both fundamental and translational research.
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