[Determination of polyamines in various biological media]

The existing methods for the determination of polyamines in various biological objects were assessed. The simplest, accessible, rather sensitive, and rapid method to determine polyamines, which is based on the electrophoretic fractionation of polyamines on 1.5% agar, is proposed for extensive clinical application. The proposed method has been used to study the levels of free polyamines in the spermoplasma of fertile and subfertile males. Different forms of subfertility showed significant differences in both the concentration of polyamines and their ratio as compared to those in the fertile males. The findings may be of diagnostic value in practical andrology.